Descargar Genetica Strickberger Pdf En 15

For examining within species polymorphism of the plastid genome, 150 grams =
of fresh leaf tissue was collected and pooled from multiple individuals fro=
m four populations of C. americanum (VA, MN, OH, and AL), including the sam=
e VA population used for the single population plastid sequencing (Table 1)=
. These populations were chosen because they span the geographic range of C=
. americanum and were known to differ genetically based on sequencing of in=
dividual chloroplast loci (Barnard-Kubow et al., unpublished results). VA i=
ndividuals were transplanted from the field, while MN, OH, and AL individua=
ls were germinated from field-collected seed in a growth chamber. Plants fr=
om all four populations were then grown for several months in the greenhous=
e with regular watering and fertilization. Intact chloroplasts were isolate=
d from the single population (VA) and pooled samples using a combination of=
 differential centrifugation and separation on a sucrose step gradient [19]=
,[20]. Chloroplasts were then lysed, and DNA was obtained via a phenol-chlo=
roform extraction and ethanol precipitation. The purity of plastid DNA (cpD=
NA) was confirmed by restriction digestion.

descargar genetica strickberger pdf en 15
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