Probeset/Transcript cluster definitions for HTA2.0 using pdInfoBuilder

Guilherme Rocha <[email protected]> Tue, 26 Aug 2014 10:00:36 -0400
Newsgroups gmane.science.biology.informatics.conductor
Message-ID <CAG3yinrAOktZ_-5m+TVtnd5T-uPKhZHfBnbhuSN1DBr3GopMwQ@mail.gmail.com>
  Hi all,

  I have constructed a package information file for Affy's HTA 2.0 chip
using pdInfoBuilder as shown below.
  It appears that the annotation files have been upgraded to na34 (from
na33 in probeFile and transFile).

  Specific question: do the annotation files affect which probes are
included in each probeset/trascript cluster?
  Broader question: what information from the annotation files is actually
used by pdInfoBuider?

  Any help appreciated.

  Thanks,

  Guilherme Rocha


---------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Construction fo the package:

library(pdInfoBuilder)

setwd("/my_bioc_packages/")

seed <- new("AffyHTAPDInfoPkgSeed",
            version     = "3.8.0",
            license     = "Artistic-2.0",
            pgfFile     = ".../HTA-2_0.r1.pgf",
            clfFile     = ".../HTA-2_0.r1.clf",
            probeFile   = ".../HTA-2_0.na33.hg19.probeset.csv",
            transFile   = ".../HTA-2_0.na33.1.hg19.transcript.csv",
            coreMps     = ".../HTA-2_0.r1.Psrs.mps",
            geneArray   = TRUE,
            author      = "gvrocha",
            email       = "[email protected]",
            biocViews   = "AnnotationData",
            genomebuild = "hg19",
            organism    = "Homo sapiens",
            species     = "Homo sapien",
            url         = "http://about.me/gvrocha")

makePdInfoPackage(seed, destDir=".")


-- 
Guilherme V. Rocha
[email protected]

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