HTSeq-Count
"Julia [guest]" <[email protected]> Fri, 5 Sep 2014 07:41:05 -0700 (PDT)
| Newsgroups | gmane.science.biology.informatics.conductor |
|---|---|
| Message-ID | <[email protected]> |
Hi all, I am new to the field of seq and performed a RIP-Seq experiment using HTSeq count as counter. I get now the following (using union, but doesn´t look better for interesection_strict): __no_feature 1503377 __ambiguous 490772 __too_low_aQual 0 __not_aligned 0 __alignment_not_unique 5277314 When I sum up counts for all genes, I get 3227845. The number for __no_feature, __ambiguous, __alignment_not_unique look very high. Does somebody have an idea for that? (Additional info: We did random priming and mapped with STAR and masked rRNA loci) Best wishes Julia -- output of sessionInfo(): . -- Sent via the guest posting facility at bioconductor.org. _______________________________________________ Bioconductor mailing list [email protected] https://stat.ethz.ch/mailman/listinfo/bioconductor Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor