Consensus sequence
"James W. MacDonald" <[email protected]> Mon, 8 Sep 2014 22:32:25 -0400
| Newsgroups | gmane.science.biology.informatics.conductor |
|---|---|
| Message-ID | <CAKO-U0p7QvPoys9Y2ZDTBHLs=DanFBYUv9Bq8HVsmbBEDabiBg@mail.gmail.com> |
I have a use case that I am not sure the best way to proceed. I have a non-model organism for which we would like to know the sequence for a set of genes. It's a bird species, and I can for example align to the zebra finch genome, then run bam_tally over a region to get variants where my species differs from zebra finch, and then infer the sequence based on the reference and the variants. But that seems harder than it should be. Is there some function that I am missing that I can feed a GRanges and get back the consensus sequence for that region, based on say a simple vote of the reads that overlap it? Thanks, Jim [[alternative HTML version deleted]] _______________________________________________ Bioconductor mailing list [email protected] https://stat.ethz.ch/mailman/listinfo/bioconductor Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor