CryoNet #32501 - #32504

CryoNet - Tue 23 Mar 2010

    #32501: #32497: Translation of German glassy protein paper [RAMole] [Jens Rabis]
    #32502: Re: on the "delicate and fleeting nature" of synapses [robomoon]
    #32503: Down with Uploading [Ettinger]
    #32504: An incentive to manage reanimation trusts. [MARK PLUS]

Rate This Digest: http://www.cryonet.org/cgi-bin/rate.cgi?msg=32501%2D32504

Administrivia

To subscribe to CryoNet, send email to:
    [email protected]
with the subject line (not message _body_):
    subscribe
To unsubscribe, use the subject line:
    unsubscribe
To post a message to CryoNet, send your message to:
    [email protected]
from the same address to which you are aubscribed.


----------------------------------------------------------------------

Message #32501
From: "Jens Rabis" <[email protected]>
References: <[email protected]>
Subject: #32497: Translation of German glassy protein paper [RAMole]
Date: Mon, 22 Mar 2010 12:01:03 +0100

Hi RAMole,

thanks :-)
Here is the direct comparison of "Google-Translation" and "Babel Fish".
Translated into English was the German link:
http://www.scienceticker.info/2010/03/19/protein-zu-glasperlen/

http://translate.google.de/#

An elegant method for the preservation of proteins, American researchers
have developed. By tiny drops of a protein solution into a liquid desiccant
type, transforming the droplets into beads of glass protein. 
Image: Deborah Rickard, Pratt School of Engineering, Duke University 

"This protein is non-crystalline glass," said David Needham of Duke
University, "it is rather a frozen liquid." Unlike in a crystal, the
molecules are not arranged in a glass lined up in formation. Apparently, the
dehydration successes so fast that the relatively large protein molecules
can not slip into a crystal lattice. 

In their experiments, Needham and colleagues appeared a thin pipette tip in
decanol, a relative of the long-chain alcohol drinking ethanol. Then they
pressed a tiny drop of protein solution from the tip. Within a short time
that went into the protein solution contained in the alcohol over water.
Leaving behind a few hundredths of a millimeter large pearl pure protein,
the researchers report in the "Biophysical Journal. 

The process works so carefully that in glass convicted enzymes after
re-addition of water are again operational, initial tests showed four
different proteins. The reason for this is likely to pass only the water
molecules in the vicinity of the proteins in the alcohol. Those water
molecules that are embedded in the structure of large protein molecules,
however, remain in place. 

For microbes is not this "structural water" accessible, so that the protein
glass does not constitute a breeding ground for bacteria or fungi, said
Needham colleague David Gaul. In addition, protein glass is easier to
produce and easier to use as a freeze-injected protein and could, for
example in the case of therapeutic proteins directly into the body. A patent
for their technique, the researchers have applied already. 

Research: Deborah L. Rickard, P. Brent Duncan, David Gaul and David Needham,
Department of Mechanical Engineering and Materials Science, Duke University,
Durham, North Carolina 

Publication Biophysical Journal, Vol 98 (6), pp 1075-84, DOI
10.1016/j.bpj.2009.11.043



http://babelfish.yahoo.com/


American researchers developed an elegant method for the preservation of
proteins. By giving tiny drops to a protein solution to a liquid desiccator,
the droplets transform into beads from protein glass. Picture: Deborah
Rickard, Pratt School OF engineering, Duke University "This protein glass is
not crystalline", explains David Needham of the Duke University, "it is
rather a rigid liquid." Differently than in a crystal, the molecules are in
a glass not in line up and member arranged. Obviously take place the
dehydration so rapidly that the comparatively large protein molecules no
more could not slip into a crystal lattice. With their experiments Needham
and colleagues dipped a thin pipette point into Dekanol, langkettigen
relatives of the drinking alcohol ethanol. Then they pressed a tiny drop of
a protein solution from the point. Within short time the water contained in
the protein solution changed into the alcohol. Back few Hunderstel
millimeter remained a large bead of pure protein, reports the researchers in
the "Biophysical journal". The procedure works so carefully that in glass
transferred enzymes are again functional after renewed addition of water,
resulted in first tests with four different proteins. The reason for the
fact might be that only the water molecules change into the environment of
the proteins into the alcohol. Those water molecules, which are embedded
into the structure of the large protein molecules, remain against it at
their place. For microbes this "structural water" is not accessible, so that
the protein glass does not represent fertile soil for bacteria or mushrooms,
describes Needhams colleague David Gaul. Besides protein glass is easier to
manufacture and more simply manageable than freezingdried protein and can,
approximately in case of therapeutic proteins, directly into the body be
injected. The researchers already requested a patent on their technology.
Research: Deborah L. Rickard, P. Brent Duncan, David Gaul and David Needham,
department OF Mechanical engineering and of material Science, Duke
University, Durham, North Carolina Publication Biophysical journal, VOL. 98
(6), pp 1075-84, DOI 10.1016/j.bpj.2009.11.043

Best  greetings
Jens  Rabis
Germany-Berlin





Message #32497
From: [email protected]
Date: Sun, 21 Mar 2010 12:40:30 EDT
Subject: Translation of German glassy protein paper

Content-Language: en

Hello all:
 
Here is an on-line translation of the  paper pasted here in German. The 
translation is pretty good in spots and  otherwise it's amusing. It gives a 
good idea of the current state of translation  programs (and would probably
do 
much better on non-technical material.) The  original text is below so those

who read German better than I can make  comparisons.
 
__________________________
 
An elegant method for preserving  EiweiBen have American
Researchers developed. By tiny drops of a  protein solution into a
liquid super desiccant type transform  the droplet into beads
Protein glass.

"This  glass is not crystalline protein," explains David Needham of the
Duke  University, "it is rather a frozen liquid." Unlike in
a  crystal, the molecules are in a jar is not in the ranks
arranged. Apparently, the  dehydration successes so quickly that the
relatively  coarse Proteinmolekule no longer in a crystal lattice
could slip.

In their  experiments, Needham and colleagues appeared a thin
Pipette tip in decanol, a long-chain  relatives of drinking alcohol
Ethanol. Then  they printed out a tiny drop of protein solution
the top. Within a  short time that went into the protein solution contained
About water in the alcohol. Back stayed a few  hundredths of a millimeter
rough  pearl pure protein, the researchers report in the "Biophysical
Journal.

The  process works so carefully that in glass uberfuhrte enzymes  after
further  addition of water are again funktionstuchtig, initial tests  showed
with four different proteins. The reason for this could be  that only the
Water molecules in the vicinity of  the proteins ubergehen in alcohol. Those
Water molecules that are embedded in  the structure of the coarse 
Proteinmolekule
are, however, remain in  place.

For  microbes is this "structural water" is not accessible, so that  the
Protein  not Glass Nahrboden for bacteria or fungi constitutes explained
Needham colleague David Gaul. In addition, protein  glass is easier to 
produce and
easier to use as a freeze-dried  protein and konne, such as
The  case of therapeutic EiweiBe be injected directly into the body. A
Patent on their technique, the  researchers have applied already.

Research:  Deborah L. Rickard, P. Brent Duncan, David and David Gaul
Needham, Department of Mechanical  Engineering and Materials Science, Duke
University, Durham, North  Carolina

Publication from Biophysical Journal,  Vol 98 (6), pp 1075-84, DOI
10.1016/j.bpj.2009.11.043 "End of  quote, source:



In a message dated 3/21/2010 3:00:25 A.M. Mountain Daylight Time,  
[email protected] writes:


Message  #32495
From: "Jens Rabis"  <[email protected]>
References:  <[email protected]>
Subject: AW: CryoNet #32492  - #32494
Date: Sat, 20 Mar 2010 18:02:00  +0100

Betreff:

Message #32494
Date: Fri, 19 Mar 2010 19:53:11  -0800 (PST)
From: [email protected]
Subject: New Technique Turns Proteins  Into Glass

****************************

Information in  German:


Zitat: " Protein zu Glasperlen 19. Marz 2010 12:17  

Eine elegante Methode zur Konservierung von EiweiBen haben  amerikanische
Forscher entwickelt. Indem sie winzige Tropfen einer  Proteinlosung in ein
flussiges Trockenmittel geben, verwandeln sich die  Tropfchen in Perlen aus
Proteinglas. 

"Dieses Proteinglas ist nicht  kristallin", erklart David Needham von der
Duke University, "es ist  vielmehr eine erstarrte Flussigkeit." Anders als 
in
einem Kristall, seien  die Molekule in einem Glas nicht in Reih und Glied
angeordnet. Offenbar  erfolge der Wasserentzug so rasch, dass die
vergleichsweise groBen  Proteinmolekule nicht mehr in ein Kristallgitter
rutschen  konnten.

Bei ihren Experimenten tauchten Needham und Kollegen eine  dunne
Pipettenspitze in Dekanol, einen langkettigen Verwandten des  Trinkalkohols
Ethanol. Dann druckten sie einen winzigen Tropfen einer  Proteinlosung aus
der Spitze. Binnen kurzer Zeit ging das in der  Proteinlosung enthaltene
Wasser in den Alkohol uber. Zuruck blieb eine  wenige Hunderstel Millimeter
groBe Perle reinen Proteins, berichten die  Forscher im "Biophysical
Journal".

Das Verfahren arbeitet so  schonend, dass in Glas uberfuhrte Enzyme nach
erneuter Zugabe von Wasser  wieder funktionstuchtig sind, ergaben erste 
Tests
mit vier verschiedenen  Proteinen. Der Grund dafur durfte sein, dass nur die
Wassermolekule in der  Umgebung der Proteine in den Alkohol ubergehen. Jene
Wassermolekule, die in  die Struktur der groBen Proteinmolekule eingebettet
sind, bleiben dagegen  an ihrem Platz.

Fur Mikroben sei dieses "Strukturwasser" nicht  zuganglich, sodass das
Proteinglas keinen Nahrboden fur Bakterien oder  Pilze darstelle, erlautert
Needhams Kollege David Gaul. Zudem sei  Proteinglas leichter herzustellen 
und
einfacher handhabbar als  gefriergetrocknetes Protein und konne, etwa im
Falle therapeutischer  EiweiBe, direkt in den Korper injiziert werden. Ein
Patent auf ihre Technik  haben die Forscher bereits beantragt.

Forschung: Deborah L. Rickard, P.  Brent Duncan, David Gaul und David
Needham, Department of Mechanical  Engineering and Materials Science, Duke
University, Durham, North  Carolina

Veroffentlichung Biophysical Journal, Vol. 98(6), pp 1075-84,  DOI
10.1016/j.bpj.2009.11.043"  Zitat Ende,  Quelle:


http://www.scienceticker.info/2010/03/19/protein-zu-glasperlen/



Best  greetings
Jens  Rabis
Germany-Berlin




 Content-Type: text/html; charset="UTF-8"

[ AUTOMATICALLY SKIPPING HTML ENCODING! ] 

Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=32497

Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=32501

----------------------------------------------------------------------

Message #32502
Date: Mon, 22 Mar 2010 16:19:54 +0100 (CET)
From: [email protected]
Subject: Re: on the "delicate and fleeting nature" of synapses
References: <[email protected]>

Dear friends, true words block a clouded vision. When it's funny, don't rate this message as poor. The telegraph.co.uk article from 17 Mar 2010 is called: medieval child's brain to unlock human thought processes. See, there we got it! They had witch potions 800 years ago, that's probably the magic brew where bacteria made the enzymes reacting with body fats, water, and hydrogen, producing a soap-like substance to preserve some neurons and other tiny things in that brain.

They also had powerful wizards 800 years ago in Quimper, Brittany, not far from the location where the grave was excavated 1998. So Christina Papageorgopoulou might have written the Neuroimage article under the influence of an evil spell from Gaulish druids and the 18 years old boy was probably a bewitched prince, trying to break the unblemished sciences with spiritual forces in a single (and therefore paranormal) brain preservation that occurred despite the absence of cryogenic temperatures. But beware, there's an 8 in the above 3 numbers.

Frank Ruhli, head of the Swiss Mummy Project at the University of Zurich, has already hosted holy majesties like the great Egyptian King Tutankhamun who are likely now to seek revenge for their plundered graves. See, there's a secret omen too! So stay away from an alchemy like this, nothing good will come over us when the curse of the mummy's tomb strikes the outlawed.

>     #32498: on the "delicate and fleeting nature" of synapses [Jeff Davis]
> Message #32498
> Date: Sun, 21 Mar 2010 11:18:40 -0700
> Subject: on the "delicate and fleeting nature" of synapses
> From: Jeff Davis <[email protected]>
> 
> "The almost perfectly preserved brain of a medieval toddler who died
> 800 years ago..."
> 
> "...was exhumed in 1998 and after more than a decade of research
> scientists have now identified neurons and cerebral cells that are
> still intact.
> 
> http://www.telegraph.co.uk/news/worldnews/europe/france/7464185/Medieval-chi
> lds-brain-to-unlock-human-thought-processes.html
> 
> Best, Jeff Davis
//



Topp oder Hopp? Tolle Figur, scharfes Dekollete oder sehenswertes Tatoo?! Bewerten Sie die besten Bilder und zeigen Sie auch selbst, was Sie haben! Jetzt reinklicken und mitmachen: http://www.arcor.de/rd/footer.toh

Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=32502

----------------------------------------------------------------------

Message #32503
From: [email protected]
Date: Mon, 22 Mar 2010 13:12:05 EDT
Subject: Down with Uploading

Content-Language: en

 
The  relevance of this to cryonics is marginal but not zero. It is 
political or  PR-related.  
I  think there is a small but not insignificant group who think that, since 
quite a  few 
cryonicists are also Uploaders, they are tarred with the  same brush. 
This  was instigated by my recent second looks at some works by uploaders,  
including 
Perry, Tipler, Moravec, and Kurzweil. 
Curiously, I have a lot of respect for the first two, but  not much"in this 
context"for 
the  latter two. Mike Perry, in particular, I believe thoroughly 
understands my  arguments 
against the uploading thesis. He isn't dogmatic, admits  he might be wrong, 
and has 
many  splendid things to say, such as early in Forever for All that what we 
should  aim 
for  in life is a preponderance of satisfaction over dissatisfaction"the 
meat of  my 
book  Youniverse. Cosmologist FrankTipler  makes bolder and more dogmatic 
statements,  
in  The Physics of Immortality, some of  which I consider clearly wrong, 
but still offers  
good  food for thought. Moravec and Kurzweil, on the other hand, I think 
make  too 
many  obvious blunders and are basically lightweights in this area. 
So  let me attempt in extreme brevity first to list some of the 
shortcomings of  the 
Uploading thesis. If there is any interest, I could  follow this with more 
detail. 
It's  all old hat, of course, but there are always new people and old 
people  with 
new  interests. These notes are in no particular order. 
"Identity of indiscernibles" is a  common tenet. Often attributed to 
Leibniz,  
one  version is that if two physical objects or systems cannot be  
distinguished 
from  each other by any criterion, then they  must be considered the "same"
 
or  identical.  
First, this assertion actually asserts nothing except a  certain preference 
in 
use  of language. It has no consequences. It is also useless because if  
the 
question arises, are A and B distinguishable, the answer  is always yes. An 
 
example might be two hydrogen atoms, which some would say  fill the 
bill. But the atoms at minimum are at different  locations, hence are 
distinguishable, e.g. by a mass detector of appropriate  sensitivity. In 
addition, being at different locations, they necessarily  differ in other 
ways  too, such as the gravitational fields to which they are subjected. 
The sufficiency of isomorphism is a  pervasive and clearly wrong 
idea. Isomorphism means roughly same-in-form, and refers  most 
commonly to the fact that, in a computer simulation of a  physical 
object or system, every attribute of the original has a  counterpart 
in  the simulation, and it is therefore allowable to think that the 
simulation is "just as good" as the original and that a  simulated 
person would be alive and conscious. 
Among other fatal afflictions, which I have spelled out  in Youniverse,  
this  idea simply assumes, without proof, that a description or  
representation of a thing is the "same" as the thing. In  a few limited  
circumstances this is true, the most obvious example  being a map.  
If  two maps show the same city with equal fidelity, the maps are  
essentially the same and either can be used. But no map  is the city.  
The  map is not the territory, and a description of a person, no matter  
how  detailed and whether or not dynamic, is not the person. 
For  an easily understood reductio ad  absurdum, consider a 
description of a hydrogen atom, in its ground state and  far 
removed from all other influences. Anyone"even  I"could 
write down, with pencil and paper, words and  numbers 
providing a quantum mechanical description of the  atom, 
its  wave equation or equivalent. But no one will claim, I 
hope, that the pencil marks on paper CONSTITUTE a  hydrogen 
atom. Yet this is exactly, in effect, what the Uploaders  do 
claim. There is no difference, in principle, between a  computer 
simulation of a hydrogen atom and my pencil marks  on 
paper. In either case we just have a coded description  of 
a  physical system, which has to be interpreted.  
Enough for now. If there are any gluttons for punishment,  I 
can  continue. 
Robert Ettinger


 Content-Type: text/html; charset="UTF-8"

[ AUTOMATICALLY SKIPPING HTML ENCODING! ] 

Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=32503

----------------------------------------------------------------------

Message #32504
Date: Mon, 22 Mar 2010 22:16:50 -0700
Subject: An incentive to manage reanimation trusts.
From: MARK PLUS <[email protected]>

The Anticult on Rick Ross's Cult Education Forum seems stuck on the
idea that the people nominated as trustees of a cryonicist's
reanimation fund would immediately steal the money after the
cryonicist dies.

Dr. Steve Harris counters that this almost never happens to all kinds
of posthumous trusts in the real world.

So why would that happen to reanimation trusts? Trustees of fortunes
which don't belong to them gain status by showing that they don't
abuse their positions. An honest trustee signals to other people that
he has integrity and good character, even if the trust he manages and
protects has some unusual but defensible purpose.


--
Mark Plus
Life is short: Freeze hard!

Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=32504

----------------------------------------------------------------------

End of CryoNet Digest
*********************