CryoNet #32497 - #32500

CryoNet - Mon 22 Mar 2010

    #32497: Translation of German glassy protein paper [RAMole]
    #32498: on the "delicate and fleeting nature" of synapses [Jeff Davis]
    #32499: Re: Alcor-lovers vs. Neuro-lovers  [Mike Perry]
    #32500: Re: Alcor-lovers vs. Neuro-lovers  [Mike Perry]

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Message #32497
From: [email protected]
Date: Sun, 21 Mar 2010 12:40:30 EDT
Subject: Translation of German glassy protein paper

Content-Language: en

Hello all:
 
Here is an on-line translation of the  paper pasted here in German. The 
translation is pretty good in spots and  otherwise it's amusing. It gives a 
good idea of the current state of translation  programs (and would probably do 
much better on non-technical material.) The  original text is below so those 
who read German better than I can make  comparisons.
 
__________________________
 
An elegant method for preserving  EiweiBen have American
Researchers developed. By tiny drops of a  protein solution into a
liquid super desiccant type transform  the droplet into beads
Protein glass.

"This  glass is not crystalline protein," explains David Needham of the
Duke  University, "it is rather a frozen liquid." Unlike in
a  crystal, the molecules are in a jar is not in the ranks
arranged. Apparently, the  dehydration successes so quickly that the
relatively  coarse Proteinmolekule no longer in a crystal lattice
could slip.

In their  experiments, Needham and colleagues appeared a thin
Pipette tip in decanol, a long-chain  relatives of drinking alcohol
Ethanol. Then  they printed out a tiny drop of protein solution
the top. Within a  short time that went into the protein solution contained
About water in the alcohol. Back stayed a few  hundredths of a millimeter
rough  pearl pure protein, the researchers report in the "Biophysical
Journal.

The  process works so carefully that in glass uberfuhrte enzymes  after
further  addition of water are again funktionstuchtig, initial tests  showed
with four different proteins. The reason for this could be  that only the
Water molecules in the vicinity of  the proteins ubergehen in alcohol. Those
Water molecules that are embedded in  the structure of the coarse 
Proteinmolekule
are, however, remain in  place.

For  microbes is this "structural water" is not accessible, so that  the
Protein  not Glass Nahrboden for bacteria or fungi constitutes explained
Needham colleague David Gaul. In addition, protein  glass is easier to 
produce and
easier to use as a freeze-dried  protein and konne, such as
The  case of therapeutic EiweiBe be injected directly into the body. A
Patent on their technique, the  researchers have applied already.

Research:  Deborah L. Rickard, P. Brent Duncan, David and David Gaul
Needham, Department of Mechanical  Engineering and Materials Science, Duke
University, Durham, North  Carolina

Publication from Biophysical Journal,  Vol 98 (6), pp 1075-84, DOI
10.1016/j.bpj.2009.11.043 "End of  quote, source:



In a message dated 3/21/2010 3:00:25 A.M. Mountain Daylight Time,  
[email protected] writes:


Message  #32495
From: "Jens Rabis"  <[email protected]>
References:  <[email protected]>
Subject: AW: CryoNet #32492  - #32494
Date: Sat, 20 Mar 2010 18:02:00  +0100

Betreff:

Message #32494
Date: Fri, 19 Mar 2010 19:53:11  -0800 (PST)
From: [email protected]
Subject: New Technique Turns Proteins  Into Glass

****************************

Information in  German:


Zitat: " Protein zu Glasperlen 19. Marz 2010 12:17  

Eine elegante Methode zur Konservierung von EiweiBen haben  amerikanische
Forscher entwickelt. Indem sie winzige Tropfen einer  Proteinlosung in ein
flussiges Trockenmittel geben, verwandeln sich die  Tropfchen in Perlen aus
Proteinglas. 

"Dieses Proteinglas ist nicht  kristallin", erklart David Needham von der
Duke University, "es ist  vielmehr eine erstarrte Flussigkeit." Anders als 
in
einem Kristall, seien  die Molekule in einem Glas nicht in Reih und Glied
angeordnet. Offenbar  erfolge der Wasserentzug so rasch, dass die
vergleichsweise groBen  Proteinmolekule nicht mehr in ein Kristallgitter
rutschen  konnten.

Bei ihren Experimenten tauchten Needham und Kollegen eine  dunne
Pipettenspitze in Dekanol, einen langkettigen Verwandten des  Trinkalkohols
Ethanol. Dann druckten sie einen winzigen Tropfen einer  Proteinlosung aus
der Spitze. Binnen kurzer Zeit ging das in der  Proteinlosung enthaltene
Wasser in den Alkohol uber. Zuruck blieb eine  wenige Hunderstel Millimeter
groBe Perle reinen Proteins, berichten die  Forscher im "Biophysical
Journal".

Das Verfahren arbeitet so  schonend, dass in Glas uberfuhrte Enzyme nach
erneuter Zugabe von Wasser  wieder funktionstuchtig sind, ergaben erste 
Tests
mit vier verschiedenen  Proteinen. Der Grund dafur durfte sein, dass nur die
Wassermolekule in der  Umgebung der Proteine in den Alkohol ubergehen. Jene
Wassermolekule, die in  die Struktur der groBen Proteinmolekule eingebettet
sind, bleiben dagegen  an ihrem Platz.

Fur Mikroben sei dieses "Strukturwasser" nicht  zuganglich, sodass das
Proteinglas keinen Nahrboden fur Bakterien oder  Pilze darstelle, erlautert
Needhams Kollege David Gaul. Zudem sei  Proteinglas leichter herzustellen 
und
einfacher handhabbar als  gefriergetrocknetes Protein und konne, etwa im
Falle therapeutischer  EiweiBe, direkt in den Korper injiziert werden. Ein
Patent auf ihre Technik  haben die Forscher bereits beantragt.

Forschung: Deborah L. Rickard, P.  Brent Duncan, David Gaul und David
Needham, Department of Mechanical  Engineering and Materials Science, Duke
University, Durham, North  Carolina

Veroffentlichung Biophysical Journal, Vol. 98(6), pp 1075-84,  DOI
10.1016/j.bpj.2009.11.043"  Zitat Ende,  Quelle:


http://www.scienceticker.info/2010/03/19/protein-zu-glasperlen/



Best  greetings
Jens  Rabis
Germany-Berlin




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Message #32498
Date: Sun, 21 Mar 2010 11:18:40 -0700
Subject: on the "delicate and fleeting nature" of synapses
From: Jeff Davis <[email protected]>

"The almost perfectly preserved brain of a medieval toddler who died
800 years ago..."

"...was exhumed in 1998 and after more than a decade of research
scientists have now identified neurons and cerebral cells that are
still intact.

http://www.telegraph.co.uk/news/worldnews/europe/france/7464185/Medieval-childs-brain-to-unlock-human-thought-processes.html

Best, Jeff Davis

 "Death is really just an engineering problem."
                       Regina Pancake

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Message #32499
Date: Sun, 21 Mar 2010 23:48:09 -0700
From: Mike Perry <[email protected]>
Subject: Re: Alcor-lovers vs. Neuro-lovers 
References: <[email protected]>

>Message #32493
>From: David Stodolsky <[email protected]>
>
>[...]
>Since neither ALCOR nor CI will cooperate in making even existing data
>available that could shed some light on these issues, the only
>evidence you are likely to get is from a government official pounding
>on the door.
>
>
>dss

I'm sorry you had the problems you had, and I wish I had been aware 
of this at the time it was happening. If you are still interested get 
in touch, tell me what you want, and I'll see what I can do.

Mike Perry

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Message #32500
Date: Sun, 21 Mar 2010 23:48:09 -0700
From: Mike Perry <[email protected]>
Subject: Re: Alcor-lovers vs. Neuro-lovers 
References: <[email protected]>

>Message #32493
>From: David Stodolsky <[email protected]>
>
>[...]
>Since neither ALCOR nor CI will cooperate in making even existing data
>available that could shed some light on these issues, the only
>evidence you are likely to get is from a government official pounding
>on the door.
>
>
>dss

I'm sorry you had the problems you had, and I wish I had been aware 
of this at the time it was happening. If you are still interested get 
in touch, tell me what you want, and I'll see what I can do.

Mike Perry

Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=32500

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