CryoNet #32593

CryoNet - Wed 26 May 2010

    #32593: Brain plastination. Free publication [Eisab]

Rate This Digest: http://www.cryonet.org/cgi-bin/rate.cgi?msg=32593%2D32593

Administrivia

To subscribe to CryoNet, send email to:
    [email protected]
with the subject line (not message _body_):
    subscribe
To unsubscribe, use the subject line:
    unsubscribe
To post a message to CryoNet, send your message to:
    [email protected]
from the same address to which you are aubscribed.
Send questions, comments, or feedback to [email protected]
with "CryoNet" or "cryonics" somewhere in the Subject line.


----------------------------------------------------------------------

Message #32593
From: "Eisab" <[email protected]>
References: <[email protected]>
Subject: Brain plastination. Free publication
Date: Tue, 25 May 2010 16:28:30 -0400

http://www.journal.plastination.org/archive/jp_vol.14.1/jp_vol.14.1_22-24.pdf

One human brain was used for this study. The brain was fixed in 5% formalin 
for two months, rinced and cut in two halves on the

sagital plane. Both brain halves were sagitally sliced at a tickness of 4 
mm. From each brain half we selected 8 slices and plastinated them

with P40 using different immersion and impregnation conditions. Two points 
were marked on each slice and subsequently an imprint of

the slices was drawn on transparency film. After dehydration in -25 C 
acetone, the slices of the left brain half were immersed at -25 C,

for two days in P40 and then impregnated for 24 hours. The slices of the 
right brain half were immersed at +5 C for two days and

impregnated at room temperature at+15 C for 24 hours. All impregnated slices 
were cured with UV light. The imprints of the fixed brain

slices were scanned into a computer, as well as the plastinated slices. By 
using a Kontron KSA 400 v. 2.0 (ZEISS) software we calculated

the area of the plastinated brain slices as well as the area of the scanned 
imprints. By comparing the obtained data we were able to

determine the shrinkage rate of the slices. The slices processed at -25 C 
showed a shrinkage rate of 4.41%. In comparison the slices

immersed at +5 C and impregnated at +15 C showed a shrinkage rate of 6.96%

Rate This Message: http://www.cryonet.org/cgi-bin/rate.cgi?msg=32593

----------------------------------------------------------------------

End of CryoNet Digest
*********************