[dinosaur] Hadrosaur skin with 3D preservation (free pdf)

Ben Creisler <[email protected]> Tue, 31 Dec 2019 08:09:43 -0800
Newsgroups gmane.science.dinosaurs.general
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Ben [email protected]=0A=0AA new paper with free pdf:=0A=0AMat=
teo Fabbri,  Jasmina Wiemann,  Fabio Manucci & Derek E. G. Briggs (2019)=0A=
Three=E2=80=90dimensional soft tissue preservation revealed in the skin of =
a=0Anon=E2=80=90avian dinosaur.=0APalaeontology (advance online publication=
)=0Adoi:  https://urldefense.proofpoint.com/v2/url?u=3Dhttps-3A__doi.org_10=
.1111_pala.12470&d=3DDwIFaQ&c=3DclK7kQUTWtAVEOVIgvi0NU5BOUHhpN0H8p7CSfnc_gI=
&r=3DRy_mO4IFaUmGof_Yl9MyZgecRCKHn5g4z1CYJgFW9SI&m=3DvCozh0pMCUdSu291HAXhXw=
OQMZdqGGu0z5ujJVAvvqE&s=3DGtbA3tInIsADQOKiwL71wY0_2wfl9qA5GIdCuBUe8WU&e=3D=
=20=0Ahttps://urldefense.proofpoint.com/v2/url?u=3Dhttps-3A__onlinelibrary.=
wiley.com_doi_full_10.1111_pala.12470&d=3DDwIFaQ&c=3DclK7kQUTWtAVEOVIgvi0NU=
5BOUHhpN0H8p7CSfnc_gI&r=3DRy_mO4IFaUmGof_Yl9MyZgecRCKHn5g4z1CYJgFW9SI&m=3Dv=
Cozh0pMCUdSu291HAXhXwOQMZdqGGu0z5ujJVAvvqE&s=3Dj7Dcq-P9M_iR340hUIVQC0rMuyKy=
Ln_ALSWp80DdA7Q&e=3D=20=0A=0AFree pdf:=0Ahttps://urldefense.proofpoint.com/=
v2/url?u=3Dhttps-3A__onlinelibrary.wiley.com_doi_pdf_10.1111_pala.12470&d=
=3DDwIFaQ&c=3DclK7kQUTWtAVEOVIgvi0NU5BOUHhpN0H8p7CSfnc_gI&r=3DRy_mO4IFaUmGo=
f_Yl9MyZgecRCKHn5g4z1CYJgFW9SI&m=3DvCozh0pMCUdSu291HAXhXwOQMZdqGGu0z5ujJVAv=
vqE&s=3DEdM6TiN1VCK9oRJeNonNi45tiEY-h0dP7GuREoVCiZI&e=3D=20=0A=0AData archi=
ving statement:=0AData for this study are available in the Dryad Digital Re=
pository:=0Ahttps://urldefense.proofpoint.com/v2/url?u=3Dhttps-3A__doi.org_=
10.5061_dryad.3bk3j9kf1&d=3DDwIFaQ&c=3DclK7kQUTWtAVEOVIgvi0NU5BOUHhpN0H8p7C=
Sfnc_gI&r=3DRy_mO4IFaUmGof_Yl9MyZgecRCKHn5g4z1CYJgFW9SI&m=3DvCozh0pMCUdSu29=
1HAXhXwOQMZdqGGu0z5ujJVAvvqE&s=3DmLUEpblrrOtauOLRKUHEuXdR3CHIoY61tvMoLb205B=
U&e=3D=20=0A=0A=0AThe most commonly preserved soft tissues associated with =
ornithischian=0Adinosaurs are skin remains. The apparent resistance of hadr=
osaur skin to=0Adecay, and its abundance in the fossil record relative to t=
hat of other=0Atetrapods, has been attributed to factors such as thickness =
and=0Acomposition. Here we report additional intrinsic factors within hadro=
saur=0Askin: 3D=E2=80=90preserved eumelanin=E2=80=90bearing bodies, dermal =
cells and blood vessel=0Afragments in an organic matrix composed of protein=
 fossilization products.=0AThe skin is much thinner than that of living mam=
mals of similar size. It is=0Alikely that the preservation of hadrosaur ski=
n is related to the=0Aarrangement of the layers composing it.=0A=0AThe skin=
 is the largest organ in the body of any vertebrate and carries out=0Aimpor=
tant functions such as homeostasis, sensory reception and visual=0Asignalin=
g (Landmann 1986). The skin is composed of three layers: the=0Aepidermis (o=
uter), dermis and the subcutis (innermost). The epidermis is=0Acomposed of =
stratified layers of keratinized cells (the stratum corneum,=0Astratum inte=
rmedium and stratum basale) whereas the dermis is characterized=0Aby a stra=
tum spongiosum of loose collagen fibres and a lower stratum=0Acompactum of =
tightly packed, orthogonal arrays of collagen fibres (Landmann=0A1986; Coop=
er & Greenberg 1992). Colouration of the skin is generally=0Adetermined by =
melanophores and chromatophores and the structural layering=0Aof the integu=
ment (McNamara et al. 2016; Shawkey & D'Alba 2017).=0AChromatophores and me=
lanophores are usually stored at the interface between=0Athe epidermis and =
dermis in vertebrates (Prum & Torres 2003, 2004; Chang et=0Aal. 2009; McNam=
ara et al. 2016; Shawkey & D'Alba 2017). The innermost layer=0Aof the skin,=
 the subcutaneous layer, separates the integument from the rest=0Aof the bo=
dy. Integument is probably the most abundant type of soft tissue=0Ain the v=
ertebrate fossil record (Schweitzer 2011; Davis 2014) and it is=0Aproviding=
 increasingly important evidence of reptile evolution and=0Aecological dive=
rsity in the past (e.g. Martill 1995; Briggs et al. 1997;=0AMartill et al. =
2000; Bell 2012; Lindgren et al. 2013, 2014, 2018; Davis=0A2014; McNamara e=
t al. 2016, 2018; Yang et al. 2019).=0A=0AFossilized skin is preserved in t=
hree main ways: as impressions,=0Acompressions or permineralized, three=E2=
=80=90dimensional skin (Schweitzer 2011).=0AImpressions are occasionally fo=
und in association with bones or footprints=0A(e.g. Lockley et al. 2004; Pa=
ik et al. 2017). Compressions are preserved as=0Aa carbon=E2=80=90rich laye=
r surrounding bones (e.g. Briggs et al. 1997; Qiang et=0Aal. 1998; Wang et =
al. 2019). Permineralized skin is the rarest of the three=0Amodes of preser=
vation (Schweitzer 2011; Bell 2012; Davis 2014) and usually=0Aoccurs in ass=
ociation with three dimensionally preserved skeletons.=0APermineralized ski=
n was previously thought to be a high=E2=80=90fidelity=0Amineralized replic=
ation of the original organic tissue (Schweitzer 2011).=0AHowever, recent i=
nvestigations of a few exceptionally preserved examples=0Ahave challenged t=
his view by recovering original organic molecules.=0ADinosaurian integument=
 preserved as a result of early mineralization is=0Aknown in Ornithischia, =
Sauropoda and Theropoda (Briggs et al. 1997; Martill=0Aet al. 2000; Bell 20=
12; Davis 2014; McNamara et al. 2018). The preservation=0Aof integument is =
most prevalent in hadrosaurs (Bell 2012; Davis 2014).=0AHowever, the taphon=
omic processes that favour the fossilization of skin and=0Aassociated molec=
ular preservation are still poorly understood (Lyson &=0ALongrich 2011; Bel=
l 2012; Davis 2014). Previous investigations of dinosaur=0Askin were perfor=
med on the basal ceratopsian Psittacosaurus (Lingham=E2=80=90Soliar=0A2008)=
, a nodosaur (Brown et al. 2017), and coelurosaurs (Naval=C3=B3n et al.=0A2=
015; Bell et al. 2017; McNamara et al. 2018). Traces of collagenous=0Amater=
ial were found in a =E2=80=98mummified=E2=80=99 hadrosaurid from North Dako=
ta, USA=0A(Manning et al. 2009). Skin colouration patterns have been recons=
tructed in=0Aornithischian dinosaurs (Lingham=E2=80=90Soliar & Plodowski 20=
10; Vinther et al.=0A2016; Brown et al. 2017) but not in a hadrosaurid.=0A=
=0AYPMPU 016969 is a partial hadrosaur skeleton preserving skin in the flan=
k=0Aregion of the body (Fabbri et al. 2019, fig. S1). We investigated this=
=0Aspecimen with the following aims: (1) to understand its taphonomy; (2) t=
o=0Atest for soft tissue preservation and the presence of melanin; and (3) =
to=0Aconsider previous explanations for the prevalence of hadrosaur skin=0A=
preservation in the fossil record.=0A=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=0A=

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<div dir=3D"ltr"><div><br></div>Ben Creisler<div><a href=3D"mailto:bcreisle=
[email protected]">[email protected]</a></div><div><br></div><div>A new paper w=
ith free pdf:<br><div><br></div><div>Matteo Fabbri, =C2=A0Jasmina Wiemann, =
=C2=A0Fabio Manucci &amp; Derek E. G. Briggs (2019)<br>Three=E2=80=90dimens=
ional soft tissue preservation revealed in the skin of a non=E2=80=90avian =
dinosaur.<br>Palaeontology (advance online publication)<br>doi: =C2=A0<a hr=
ef=3D"https://urldefense.proofpoint.com/v2/url?u=3Dhttps-3A__doi.org_10.111=
1_pala.12470&amp;d=3DDwMFaQ&amp;c=3DclK7kQUTWtAVEOVIgvi0NU5BOUHhpN0H8p7CSfn=
c_gI&amp;r=3DRy_mO4IFaUmGof_Yl9MyZgecRCKHn5g4z1CYJgFW9SI&amp;m=3DvCozh0pMCU=
dSu291HAXhXwOQMZdqGGu0z5ujJVAvvqE&amp;s=3DGtbA3tInIsADQOKiwL71wY0_2wfl9qA5G=
IdCuBUe8WU&amp;e=3D">https://doi.org/10.1111/pala.12470</a><br><a href=3D"h=
ttps://urldefense.proofpoint.com/v2/url?u=3Dhttps-3A__onlinelibrary.wiley.c=
om_doi_full_10.1111_pala.12470&amp;d=3DDwMFaQ&amp;c=3DclK7kQUTWtAVEOVIgvi0N=
U5BOUHhpN0H8p7CSfnc_gI&amp;r=3DRy_mO4IFaUmGof_Yl9MyZgecRCKHn5g4z1CYJgFW9SI&=
amp;m=3DvCozh0pMCUdSu291HAXhXwOQMZdqGGu0z5ujJVAvvqE&amp;s=3Dj7Dcq-P9M_iR340=
hUIVQC0rMuyKyLn_ALSWp80DdA7Q&amp;e=3D">https://onlinelibrary.wiley.com/doi/=
full/10.1111/pala.12470</a></div><div><br></div><div>Free pdf:<br><a href=
=3D"https://urldefense.proofpoint.com/v2/url?u=3Dhttps-3A__onlinelibrary.wi=
ley.com_doi_pdf_10.1111_pala.12470&amp;d=3DDwMFaQ&amp;c=3DclK7kQUTWtAVEOVIg=
vi0NU5BOUHhpN0H8p7CSfnc_gI&amp;r=3DRy_mO4IFaUmGof_Yl9MyZgecRCKHn5g4z1CYJgFW=
9SI&amp;m=3DvCozh0pMCUdSu291HAXhXwOQMZdqGGu0z5ujJVAvvqE&amp;s=3DEdM6TiN1VCK=
9oRJeNonNi45tiEY-h0dP7GuREoVCiZI&amp;e=3D">https://onlinelibrary.wiley.com/=
doi/pdf/10.1111/pala.12470</a><br></div><div><br>Data archiving statement:<=
br>Data for this study are available in the Dryad Digital Repository: <a hr=
ef=3D"https://urldefense.proofpoint.com/v2/url?u=3Dhttps-3A__doi.org_10.506=
1_dryad.3bk3j9kf1&amp;d=3DDwMFaQ&amp;c=3DclK7kQUTWtAVEOVIgvi0NU5BOUHhpN0H8p=
7CSfnc_gI&amp;r=3DRy_mO4IFaUmGof_Yl9MyZgecRCKHn5g4z1CYJgFW9SI&amp;m=3DvCozh=
0pMCUdSu291HAXhXwOQMZdqGGu0z5ujJVAvvqE&amp;s=3DmLUEpblrrOtauOLRKUHEuXdR3CHI=
oY61tvMoLb205BU&amp;e=3D">https://doi.org/10.5061/dryad.3bk3j9kf1</a><br><b=
r><br>The most commonly preserved soft tissues associated with ornithischia=
n dinosaurs are skin remains. The apparent resistance of hadrosaur skin to =
decay, and its abundance in the fossil record relative to that of other tet=
rapods, has been attributed to factors such as thickness and composition. H=
ere we report additional intrinsic factors within hadrosaur skin: 3D=E2=80=
=90preserved eumelanin=E2=80=90bearing bodies, dermal cells and blood vesse=
l fragments in an organic matrix composed of protein fossilization products=
. The skin is much thinner than that of living mammals of similar size. It =
is likely that the preservation of hadrosaur skin is related to the arrange=
ment of the layers composing it.<br><br>The skin is the largest organ in th=
e body of any vertebrate and carries out important functions such as homeos=
tasis, sensory reception and visual signaling (Landmann 1986). The skin is =
composed of three layers: the epidermis (outer), dermis and the subcutis (i=
nnermost). The epidermis is composed of stratified layers of keratinized ce=
lls (the stratum corneum, stratum intermedium and stratum basale) whereas t=
he dermis is characterized by a stratum spongiosum of loose collagen fibres=
 and a lower stratum compactum of tightly packed, orthogonal arrays of coll=
agen fibres (Landmann 1986; Cooper &amp; Greenberg 1992). Colouration of th=
e skin is generally determined by melanophores and chromatophores and the s=
tructural layering of the integument (McNamara et al. 2016; Shawkey &amp; D=
&#39;Alba 2017). Chromatophores and melanophores are usually stored at the =
interface between the epidermis and dermis in vertebrates (Prum &amp; Torre=
s 2003, 2004; Chang et al. 2009; McNamara et al. 2016; Shawkey &amp; D&#39;=
Alba 2017). The innermost layer of the skin, the subcutaneous layer, separa=
tes the integument from the rest of the body. Integument is probably the mo=
st abundant type of soft tissue in the vertebrate fossil record (Schweitzer=
 2011; Davis 2014) and it is providing increasingly important evidence of r=
eptile evolution and ecological diversity in the past (e.g. Martill 1995; B=
riggs et al. 1997; Martill et al. 2000; Bell 2012; Lindgren et al. 2013, 20=
14, 2018; Davis 2014; McNamara et al. 2016, 2018; Yang et al. 2019).<br><br=
>Fossilized skin is preserved in three main ways: as impressions, compressi=
ons or permineralized, three=E2=80=90dimensional skin (Schweitzer 2011). Im=
pressions are occasionally found in association with bones or footprints (e=
.g. Lockley et al. 2004; Paik et al. 2017). Compressions are preserved as a=
 carbon=E2=80=90rich layer surrounding bones (e.g. Briggs et al. 1997; Qian=
g et al. 1998; Wang et al. 2019). Permineralized skin is the rarest of the =
three modes of preservation (Schweitzer 2011; Bell 2012; Davis 2014) and us=
ually occurs in association with three dimensionally preserved skeletons. P=
ermineralized skin was previously thought to be a high=E2=80=90fidelity min=
eralized replication of the original organic tissue (Schweitzer 2011). Howe=
ver, recent investigations of a few exceptionally preserved examples have c=
hallenged this view by recovering original organic molecules. Dinosaurian i=
ntegument preserved as a result of early mineralization is known in Ornithi=
schia, Sauropoda and Theropoda (Briggs et al. 1997; Martill et al. 2000; Be=
ll 2012; Davis 2014; McNamara et al. 2018). The preservation of integument =
is most prevalent in hadrosaurs (Bell 2012; Davis 2014). However, the tapho=
nomic processes that favour the fossilization of skin and associated molecu=
lar preservation are still poorly understood (Lyson &amp; Longrich 2011; Be=
ll 2012; Davis 2014). Previous investigations of dinosaur skin were perform=
ed on the basal ceratopsian Psittacosaurus (Lingham=E2=80=90Soliar 2008), a=
 nodosaur (Brown et al. 2017), and coelurosaurs (Naval=C3=B3n et al. 2015; =
Bell et al. 2017; McNamara et al. 2018). Traces of collagenous material wer=
e found in a =E2=80=98mummified=E2=80=99 hadrosaurid from North Dakota, USA=
 (Manning et al. 2009). Skin colouration patterns have been reconstructed i=
n ornithischian dinosaurs (Lingham=E2=80=90Soliar &amp; Plodowski 2010; Vin=
ther et al. 2016; Brown et al. 2017) but not in a hadrosaurid.<br><br>YPMPU=
 016969 is a partial hadrosaur skeleton preserving skin in the flank region=
 of the body (Fabbri et al. 2019, fig. S1). We investigated this specimen w=
ith the following aims: (1) to understand its taphonomy; (2) to test for so=
ft tissue preservation and the presence of melanin; and (3) to consider pre=
vious explanations for the prevalence of hadrosaur skin preservation in the=
 fossil record.<br>=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D=3D<br></div></div></div>=
=0A=

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